Automated, Multiplexed Co-detection of RNA and Protein Julia Jones Scientific manager, Histopathology/ISH Core Facility, Cancer Research UK Cambridge Institute, University of Cambridge Co-detection of more than one target in Formalin-Fixed Paraffin Embedded samples is an extremely useful application and one that is increasingly required in modern histopathology labs. Nowadays, there is a big drive and focus in the research histopathology area towards multiplexing. Multiplexing addresses the need for researchers to assess multiple biomarkers (protein and/or nucleic acid markers) at specific locations within a tissue sample. The information revealed through simultaneous detection of multiple markers, the spatial relationships among cells and tissue in disease, and the heterogeneity are now understood to be critical to developing effective therapeutic strategies. Learning Objectives Receive an introduction to the Histopathology/ISH Core Facility at the CRUK Cambridge Institute. Understand the work carried out to date using ACD’s Integrated Co-detection Workflow (ICW) for various researchers on the BOND RX research stainer. For Research Use Only. Not for use in diagnostic procedures. Related Content Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Tips & Tricks to Multiplexing: Top 5 Reasons to Multiplex and Chromogenic versus Immunofluorescent Detection Enhanced Automated Multiplex Capabilities on Leica Biosystems BOND Research System Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only. For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Co-detection RELATED CONTENT Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research Applications Tips & Tricks to Multiplexing: Top 5 Reasons to Multiplex and Chromogenic versus Immunofluorescent Detection Enhanced Automated Multiplex Capabilities on Leica Biosystems BOND Research System Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. Apply for self-reported educational credits Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Subscreva hoje! SHARE Facebook Twitter LinkedIn Email
