Geoffrey Rolls

Download this publication to learn more about the various artifacts which may be encountered in histopathology. 

"Tissue processing” describes the steps required to take animal or human tissue from fixation to the state where it is completely infiltrated with a suitable histological wax and can be embedded ready for section cutting on the microtome.

For routine diagnosis, the use of Hematoxylin and Eosin (H&E) is by far preferred for viewing cellular and tissue structure detail by pathologists. 

Immunohistochemistry (IHC) is used in histology to detect the presence of specific protein markers that can assist with accurate tumor classification and diagnosis. This article provides an overview and the basic steps used to create an optimal IHC stain.

Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue...

Get tips for better ISH staining in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Get tips for better microtomy, flotation & section drying in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Get tips for better grossing in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

Get tips for better specimen collection and transport in this guide. Each step provides a valuable reminder of good histology practice and also helps with troubleshooting when unacceptable results do occur.

A discussion on the techniques used for removing mineral from bone, or other calcified tissue, so that high-quality paraffin sections can be prepared.