Start • Life Sciences And Research Solutions • Weiterbildung • Artikel • LS Articles • Tips & Tricks to Multiplexing: How to Choose Chromogen Colors for Multiplex and Detection Systems for Multiplex Assays RELATED PRODUCTS BOND RX Fully Automated Research Stainer BOND RXm RELATED TAGS AND TOPICS Automated IHC Workflow, Multiplex, Chromogenic Staining, Co-detection RELATED CONTENT Selecting the right detection for your IHC/ISH project (Fluorescence vs Chromogenic Staining) Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Benefits Of Automation For Tissue-Based Research Tips & Tricks to Multiplexing: How to Choose Chromogen Colors for Multiplex and Detection Systems for Multiplex Assays Rhian Evans Ph.D., Scientist Multiplexing is an important tool for simultaneous detection of multiple markers within a single tissue section. Watch our 2 short videos below to learn more about multiplexing and download the complementary infographics to keep the points on hand when you need to revisit the information! How to choose chromogen colors for multiplex If you have opted for chromogenic multiplex staining, there are additional points to consider: the choice of colors and the order of color application. While this video won’t tell you exactly what, when, and where to use different chromogens, it will provide you with some tips to consider. Check out the infographic below and learn with Larry from Leica Biosystems about the tips to consider for chromogenic multiplex staining into your tissue-based research. Image DOWNLOAD Detection systems for multiplex assays There are many decisions that must be made when adopting multiplex staining, such as choosing chromogenic or immunofluorescent multiplex assays, as well as the order and colors of chromogens and fluorophores. However, the decisions don’t stop here as there are different detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest. We will cover a few of these examples in this video. Unsure of what detection systems are available for multiplex assays? Check out the infographic below that showcases some examples of detection systems available for multiplex assays that can increase sensitivity and enhance the signal-to-noise ratio when detecting markers of interest. Image DOWNLOAD DON'T MISS OUT! SUBSCRIBE TO RESEARCH LINK TODAY TO GET QUATERLY UPDATES ON OUR LATEST LIFE SCIENCE ENDEAVOURS SUBSCRIBE For research use only. Not for use in diagnostic procedures. About the presenter Rhian Evans , Ph.D., Scientist Rhian is a Scientist from Swansea University in Medical and Healthcare Studies and was featured in several collaborative publications. Rhian’s research-based background focused on tissue-based pathology in Multiple Sclerosis, primarily using immunohistochemical analysis and in vitro molecular techniques. She spent a short period conducting routine PCR testing for COVID-19 at the end of 2020. Related Content Selecting the right detection for your IHC/ISH project (Fluorescence vs Chromogenic Staining) Design, Optimization, and Validation of Multiplex Immunofluorescence Panels Benefits Of Automation For Tissue-Based Research Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt. Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden. Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Contact Our Product Expert Now If you have viewed this educational webinar or training and would like to apply for continuing education credits with your certifying organization, please download the form to assist you in adding self-reported educational credits to your transcript. ANMELDUNG FÜR WEITERBILDUNGSPUNKTE NACH EIGENANGABEN Subscribe to our mailing list to learn about our upcoming symposiums and new research-focused product launches. Melden Sie sich jetzt an! SHARE Facebook Twitter LinkedIn Email
